Publications and References

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Wilding, Kristen M., J. Porter Hunt, Wilkerson, Parker J. Funk, Rebecca L. Swensen, William C. Carver, M. Lance Christian, and Bradley C. Bundy. Endotoxin‐free E. coli‐based cell‐free protein synthesis: Pre‐expression endotoxin removal approaches for on‐demand cancer therapeutic production. Biotech J. 2018.

Park, Ah-Reum, Seung-Won Jang, Jin-Sook Kim, Young-Gyun Park, Bong-Seong Koo, Hyeon-Cheol Lee. Efficient recovery of recombinant CRM197 expressed as inclusion bodies in E.coli. PLoS ONE 13(7): e0201060.

Rampoldi, Antonio, Stephen N. Crooke, Marcela K Preininger, Rajneesh Jha, Joshua Maxwell, Lingmei Ding, Paul Spearman, M.G. Finn, and Chunhui Xu. Targeted Elimination of Tumorigenic Human Pluripotent Stem Cells Using Suicide-inducing Virus-like Particles. ACS Chem. Biol, 2018. DOI: 10.1021/acschembio.8b00490.

Kawana, Kei and Igimi Shizunobu. Lactic-acid-bacteria-containing composition, oral pharmaceutical composition, for treating HPV infection and/or HPV-associated tumors, and mucosal immunity-inducing agent. US Patent application US2018/0008663, published January 11, 2018.

Steinmetz, Nicole F., Jonathan Pokorski, Richa A. Sitkus. Melt processed viral nanoparticle constructs. PCT Patent application WO2018/085658 published May 11, 2018.

Misra, N., T.F. Wines, C.L. Knopp, R. Hermann, L. Bond, B. Mitchell, M.A. McGuire, J.K. Tinker. Immunogenicity of a Staphylococcus aureus-cholera toxin A2/B vaccine
for bovine mastitis. Vaccine 2018.

González-Miró, Majela, Laura M. Rodríguez-Noda, Mildrey Fariñas-Medina, Barbara Cedré-Marrero, Sandra Madariaga-Zarza, Caridad Zayas-Vignier, Mabel Hernández-Cedeño, Torsten Kleffmann, Dagmar García-Rivera, Vicente Vérez-Bencomo and Bernd H. A. Rehm. Bioengineered polyester beads co-displaying protein and carbohydrate-based antigens induce protective immunity against bacterial infection Scientific Reports (2018) 8:1888. DOI:10.1038/s41598-018-20205-7.

Song, Hyun-Woo, Ji Yun Kim, Jae-Chul Pyun. Surface display of sialyltransferase on the outer membrane of Escherichia coli and ClearColi. Kor Soc Biotech Bioeng 4, 105, 2018.

Girard, Magalí C., Gonzalo R. Acevedo, Lucía López, Micaela S. Ossowski, María D. Piñeyro, Juan P. Grosso, Marisa Fernandez, Yolanda Hernández Vasquez, Carlos Robello, Karina A. Gómez. Evaluation of the immune response against Trypanosoma cruzi cytosolic Tryparedoxin Peroxidase in human natural infection. Immunology, 2018. doi: 10.1111/imm.12979

Douglas, Trevor, Benjamin H.Schwarz, Ranjit Koliyatt, Masaki Uchida and John Alexander. Presentation of Bioactive Proteins. US Patent application US2017/0321220A1 published November 9, 2017.

Norkowski, Stephanie, Britta Körner, Lilo Greune, Anne‑Sophie Stolle, Marie‑Luise Lubos,
Philip R. Hardwidge, M. Alexander Schmidt, Christian Rüter. Bacterial LPX motif‑harboring virulence factors constitute a species‑spanning family of cell‑penetrating effectors. Cell Molec Life Sci, 2017. /s000 18-017-2733 -4

Lee, Parker W., et al. “Biodegradable Viral Nanoparticle/Polymer Implants Prepared via Melt-Processing.” ACS Nano, vol. 11, no. 9, 2017, pp. 8777–8789., doi:10.1021/acsnano.7b02786

Viranaicken, Wildriss, et al. “ClearColi BL21(DE3)-Based Expression of Zika Virus Antigens Illustrates a Rapid Method of Antibody Production against Emerging Pathogens.”Biochimie, vol. 142, 2017, pp. 179–182., doi:10.1016/j.biochi.2017.09.011.

Watkins HC et al. A single dose and long lasting vaccine against pandemic influenza through the controlled release of a heterospecies tandem M2 sequence embedded within detoxified bacterial outer membrane vesicles. Vaccine (2017).

Montoliu-Gaya L, Esquerda-Canals G, BronsomsS, Villegas S (2017) Production of an anti-Aβantibody fragmentin Pichia pastoris and in vitro and in vivo validation of its therapeutic effect. PLoS ONE 12(8):e0181480.
PLoS One. 2017 v12 n8 pg1-19_Montoliu-Gaya

Rubio-Reyes, Patricia, et al. “Immunological Properties and Protective Efficacy of a Single Mycobacterial Antigen Displayed on Polyhydroxybutyrate Beads.” Microbial Biotechnology, 2017, doi:10.1111/1751-7915.12754

Lee, Parker W., et al. “Polymer Structure and Conformation Alter the Antigenicity of Virus-like Particle-Polymer Conjugates.” Journal of the American Chemical Society, vol. 139, no. 9, 2017, pp. 3312–3315., doi:10.1021/jacs.6b11643.
J Am Chem Soc. 2017 v139 n9 pg3312-5_Lee

Wang, Gaochan. “Diarrheagenic Escherichia Coli Signaling and Interactions with Host Innate Immunity and Intestinal Microbiota.” Kansas State University, 2017.
Wang G_Diarrheagenic Escherichia coli signaling … Dissertation_KansasStateU_2017

Wang, Gaochan, et al. “Enterotoxigenic Escherichia Coli Flagellin Inhibits TNF-Induced NF-ĸB Activation in Intestinal Epithelial Cells.” Pathogens, vol. 6, no. 2, 2017, p. 18., doi:10.3390/pathogens6020018.

Battin C, Hennig A, Mayrhofer P, Kunert R, Zlabinger GJ, Steinberger P, et al. (2017) A human monocytic NF-κB fluorescent reporter cell line for detection of microbial contaminants in biological samples. PLoS ONE 12(5): e0178220.

Tkachuk AP, GushchinVA, Potapov VD, DemidenkoAV, Lunin VG, GintsburgAL (2017) Multi-subunit BCG booster vaccine GamTBvac: Assessment of immunogenicity and protective efficacy in murine and guinea pig TB models.PLoS ONE 12(4):e0176784.
PloS ONE 2017 ePub Apr 28_0176784_Tkachuk

Pooe, O. J. et al., Plasmodium falciparum Heat Shock Protein 70 Lacks Immune Modulatory
Activity. Protein & Peptide Letters 24: 1-8, 2017.
Protein Pept Lett 2017 v24 n6 pg1-8_Pooe

Lee, J. W. et al., Bioengineering a bacterial pathogen to assemble its own particulate vaccine capable of inducing cellular immunity. Scientific Reports 7:41607 DOI: 10.1038/srep41607, 2017. Scientific Rep 2017 ePub 2017 Feb 2_Lee

Zhang, L. et al., Development of autologous C5 vaccine nanoparticles to reduce intravascular hemolysis in vivo. ACS Chem Biol. 2017 Feb 17;12(2):539-547. doi: 10.1021/acschembio.6b00994. Epub 2017 Jan 12. ACS Chem Biol. 2017 Jan 3 EPub_[43]_Zhang

Yoo, G., Autodisplay of the La/SSB protein on LPS-free E. coli for the diagnosis of Sjögren’s syndrome. Enzyme and Microbial Technology 100: 1–10, 2017.
Enzyme microb technol 2017 May 1 EPub_[10]_Yoo

Kim, Y. et al., Mycobacterium abscessus ESX-3 plays an important role in host inflammatory and pathological responses during infection. Microbes Infect. 19:(1) 5-17, 2017.
Microbes Infect. 2016 Sep 13 EPub_[13]_Kim

Watkins, H. C. et al., Safe recombinant outer membrane vesicles that display M2e elicit heterologous influenza protection. Mol Ther. 2017 Feb 16. pii: S1525-0016(17)30016-3. doi: 10.1016/j.ymthe.2017.01.010 Watkins Molec Ther v25 n4 2017

Schwarz, B. et al., Virus-Like Particles Encapsidating Respiratory Syncytial Virus M and M2 Proteins induce robust T cell responses. ACS Biomater. Sci. Eng. DOI: 10.1021/acsbiomaterials.6b00532 Publication Date (Web): 03 Nov 2016.
ACS Biomater. Sci. Eng 2016 Nov 8 EPub_[29]_Schwarz_Just Accepted Manuscript

Schwarz, H. et al., Biological Activity of Masked Endotoxin. Scientific Reports 7:44750 DOI: 10.1038/srep44750, 2016. Sci Rep 2017 Mar 20 srep44750_Schwarz

Dovala, D. et al., Structure-guided enzymology of the lipid A acyltransferase LpxM reveals a dual activity mechanism. Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):E6064-E6071.

Bogomolovas, J. et al., A Novel Murine Model of Parvovirus Associated Dilated Cardiomyopathy Induced by Immunization with VP1-Unique Region of Parvovirus B19. Biomed Res Intl 2016, Article ID 1627184.

Hilmann, F. et al., The Crystal Structure of Peroxiredoxin Asp f3 Provides Mechanistic Insight into Oxidative Stress Resistance and Virulence of Aspergillus fumigates. Scientific Reports 6, article number : 33396 (2016).

Reyes, P. R. et al., Immunogencity of antigens from Mycobacterium tuberculosis self-assembled as particulate vaccines. Int J Med Microbiol 2016 v306 pg624–632_Reyes

Valentine, J. L. Chen, L., Perregaux, E. C., Weyant, K. B., Rosenthal, J. A., Heiss, C., Azadi, P., Fisher, A. C., Putnam, D., Moe, G. R., Merritt, J. H. and M. P. DeLisa. Immunization with Outer Membrane Vesicles Displaying Designer Glycotopes Yields Class-Switched, Glycan-Specific Antibodies. Cell Chem. Biol. 23: 655-665, 2016.

Capsel RT, Thoen CO, Reinhardt TA, Lippolis JD, Olsen R, Stabel JR, et al. Composition and Potency Characterization of Mycobacterium avium subsp. Paratuberculosis Purified Protein Derivatives. PLoS ONE 11(5): e0154685, 2016. doi:10.1371/journal.pone.0154685

Ueda T, Akuta T, Kikuchi-Ueda T, Imaizumi K, Ono Y. Improving the soluble expression and purification of recombinant human stem cell factor (SCF) in endotoxin-free Escherichia coli by disulfide shuffling with persulfide. Protein Expr Purif. 2016 Apr;120:99-105. doi: 10.1016/j.pep.2015.12.015.

Vordenbäumen S, Saenger T, Braukmann A, Tahan T, Bleck E, Jose J, Schneider M. Human casein alpha s1 induces proinflammatory cytokine expression in monocytic cells by TLR4-signaling. Mol Nutr Food Res. 2016 Feb 22. doi: 10.1002/mnfr.201500792.

Sanchez Garcia L, Martín L, Mangues R, Ferrer Miralles N, Vázquez E, Villaverde A. Recombinant pharmaceuticals from microbial cells: a 2015 update. Microb Cell Fact 15:33, 2016.

Takafumi Ueda , Teruo Akuta, Takane Kikuchi-Ueda , Keitaro Imaizumi ,and Yasuo Ono. Improving the soluble expression and purification of recombinant human stem cell factor (SCF) in endotoxin-free Escherichia coli by disulfide shuffling with persulfide. Protein Expression and Purification 120: 99-105, 2016.

Martínez-Donato, G., Piniella, B., Aguilar, D., Olivera, S., Pérez, A., Castañedo, Y., Alvarez-Lajonchere, L., Dueñas-Carrera, S., Lee, J.W., Burr, N., Gonzalez-Miro, M., Rehm, B.H.,. Protective T cell and antibody immune response against Hepatitis C Virus using the biopolyester beads based vaccine delivery system. Clin. Vaccine Immunol. 23(4):370-8, 2016.

Karl D. Brune, Darren B. Leneghan, Iona J. Brian, Andrew S. Ishizuka, Martin F. Bachmann, Simon J. Draper, Sumi Biswas and Mark Howarth. Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization. Sci Rep. 6:19234, 2016.

Planesse C, Nativel B, Iwema T, Gasque P, Robert-Da Silva C, Viranaïcken W. Recombinant human HSP60 produced in ClearColi™ BL21(DE3) does not activate the NFκB pathway. Cytokine. 73(1):190-5, 2015.

Reading, E., Walton, T. A., Liko, I., Marty, M. T., Laganowsky, A., Rees, D. C. and C. V. Robinson. The Effect of Detergent, Temperature, and Lipid on the Oligomeric State of MscL Constructs: Insights from Mass Spectrometry. Chemistry & Biology 22, 593–603, 2015.

Mamat U, Wilke K, Bramhill D, Schromm AB, Lindner B, Kohl TA, Corchero JL, Villaverde A, Schaffer L, Head SR, Souvignier C, Meredith TC, Woodard RW. Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins. Microb Cell Fact. 14: 57, 2015. Erratum in: Microb Cell Fact.;14:81, 2015.

Wen-Sing Liang, Tristan C. Liu, Chun-Ju Chang, and Chorng-Liang Pan. Bioactivity of β-1,3-xylan Extracted from Caulerpa lentillifera by Using Escherichia coli ClearColi BL21(DE3)-β-1,3-xylanase XYLII. Journal of Food and Nutrition Research, vol. 3, no. 7 (2015): 437-444. doi: 10.12691/jfnr-3-7-5.

Fabián Rueda, Olivia Cano-Garrido, Uwe Mamat, Kathleen Wilke, Joaquin Seras-Franzoso, Elena García-Fruitós & Antonio Villaverde. Production of functional inclusion bodies in endotoxin-free Escherichia coli. Appl Microbiol Biotechnol. 98(22): 9229-38, 2014.

Hay, I. D. et al., In vivo polyester immobilized sortase for tagless protein purification. Microb Cell Fact 14:190, 2015.

Meeting Abstracts

Allikian K., C. Sun and S. Zhang. Expression of 2,6-sialyltransferase in Escherichia coli BL21(DE3) and endotoxin-free ClearColi® under fed-batch fermentation. Poster session presented at the 2016 SIMB Annual Meeting and Exhibition, New Orleans, LA. Abstract P24. July 2016.

Allikian K., I. Rasiah and S. Zhang. Effects of common defoaming agents on the growth of the endotoxin-free ClearColi® Escherichia coli host. Poster session presented at the 2016 SIMB Annual Meeting and Exhibition, New Orleans, LA. Abstract P22. July 2016.


Pooe, Oftense Jacob. Evaluation of the Immunomodulatory Activity of Plasmodium falciparum HSP70-1. Ph.D Thesis, University of Zululand, 2014. Note especially Figure 3.1