Publications and References

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Battin C, Hennig A, Mayrhofer P, Kunert R, Zlabinger GJ, Steinberger P, et al. (2017) A human monocytic NF-κB fluorescent reporter cell line for detection of microbial contaminants in biological samples. PLoS ONE 12(5): e0178220.

Pooe, O. J. et al., Plasmodium falciparum Heat Shock Protein 70 Lacks Immune Modulatory

Activity. Protein & Peptide Letters 24: 1-8, 2017.

Lee, J. W. et al., Bioengineering a bacterial pathogen to assemble its own particulate vaccine capable of

inducing cellular immunity. Scientific Reports  7:41607 DOI: 10.1038/srep41607, 2017.

Zhang, L. et al., Development of autologous C5 vaccine nanoparticles to reduce intravascular hemolysis in vivo. ACS Chem Biol. 2017 Feb 17;12(2):539-547. doi: 10.1021/acschembio.6b00994. Epub 2017 Jan 12.

Yoo, G., Autodisplay of the La/SSB protein on LPS-free E. coli for the diagnosis of Sjögren’s syndrome. Enzyme and Microbial Technology 100: 1–10, 2017.

Kim, Y. et al., Mycobacterium abscessus ESX-3 plays an important role in host inflammatory and pathological responses during infection.  Microbes Infect. 19:(1) 5-17, 2017.

Watkins, H. C. et al., Safe recombinant outer membrane vesicles that display M2e elicit heterologous

influenza protection.  Mol Ther. 2017 Feb 16. pii: S1525-0016(17)30016-3. doi: 10.1016/j.ymthe.2017.01.010

Schwarz, B. et al., Virus-Like Particles Encapsidating Respiratory Syncytial Virus M and M2 Proteins induce robust T cell responses. ACS Biomater. Sci. Eng. DOI: 10.1021/acsbiomaterials.6b00532  Publication Date (Web): 03 Nov 2016.

Schwarz, H. et al., Biological Activity of Masked Endotoxin. Scientific Reports 7:44750 DOI: 10.1038/srep44750, 2016.

Dovala, D. et al., Structure-guided enzymology of the lipid A acyltransferase LpxM reveals a dual activity mechanism. Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):E6064-E6071.

Bogomolovas, J. et al., A Novel Murine Model of Parvovirus Associated Dilated Cardiomyopathy Induced by Immunization with VP1-Unique Region of Parvovirus B19. Biomed Res Intl 2016, Article ID 1627184.

Hilmann, F. et al., The Crystal Structure of Peroxiredoxin Asp f3 Provides Mechanistic Insight into Oxidative Stress Resistance and Virulence of Aspergillus fumigates. Scientific Reports 6, article number : 33396 (2016).

Reyes, P. R. et al., Immunogencity of antigens from Mycobacterium tuberculosis self-assembled as particulate vaccines. International Journal of Medical Microbiology 306: 624–632, 2016.

Valentine, J. L. Chen, L., Perregaux, E. C., Weyant, K. B., Rosenthal, J. A., Heiss, C., Azadi, P., Fisher, A. C., Putnam, D., Moe, G. R., Merritt, J. H. and M. P. DeLisa. Immunization with Outer Membrane Vesicles Displaying Designer Glycotopes Yields Class-Switched, Glycan-Specific Antibodies. Cell Chem. Biol. 23: 655-665, 2016.

Capsel RT, Thoen CO, Reinhardt TA, Lippolis JD, Olsen R, Stabel JR, et al. Composition and Potency Characterization of Mycobacterium avium subsp. Paratuberculosis Purified Protein Derivatives. PLoS ONE 11(5): e0154685, 2016. doi:10.1371/journal.pone.0154685

Ueda T, Akuta T, Kikuchi-Ueda T, Imaizumi K, Ono Y. Improving the soluble expression and purification of recombinant human stem cell factor (SCF) in endotoxin-free Escherichia coli by disulfide shuffling with persulfide. Protein Expr Purif. 2016 Apr;120:99-105. doi: 10.1016/j.pep.2015.12.015.

Vordenbäumen S, Saenger T, Braukmann A, Tahan T, Bleck E, Jose J, Schneider M. Human casein alpha s1 induces proinflammatory cytokine expression in monocytic cells by TLR4-signaling. Mol Nutr Food Res. 2016 Feb 22. doi: 10.1002/mnfr.201500792. [Epub ahead of print]

Sanchez‑Garcia L, Martín L, Mangues R, Ferrer‑Miralles N, Vázquez E, Villaverde A. Recombinant pharmaceuticals from microbial cells: a 2015 update. Microb Cell Fact  15:33, 2016.

Takafumi Ueda , Teruo Akuta, Takane Kikuchi-Ueda , Keitaro Imaizumi ,and Yasuo Ono. Improving the soluble expression and purification of recombinant human stem cell factor (SCF) in endotoxin-free Escherichia coli by disulfide shuffling with persulfide. Protein Expression and Purification 120: 99-105, 2016.

Martínez-Donato, G., Piniella, B., Aguilar, D., Olivera, S., Pérez, A., Castañedo, Y., Alvarez-Lajonchere, L., Dueñas-Carrera, S., Lee, J.W., Burr, N., Gonzalez-Miro, M., Rehm, B.H.,. Protective T cell and antibody immune response against Hepatitis C Virus using the biopolyester beads based vaccine delivery system. Clin. Vaccine Immunol. 23(4):370-8, 2016.

Karl D. Brune, Darren B. Leneghan, Iona J. Brian, Andrew S. Ishizuka, Martin F. Bachmann, Simon J. Draper, Sumi Biswas and Mark Howarth. Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization. Sci Rep. 6:19234, 2016.

Planesse C, Nativel B, Iwema T, Gasque P, Robert-Da Silva C, Viranaïcken W. Recombinant human HSP60 produced in ClearColi™ BL21(DE3) does not activate the NFκB pathway. Cytokine. 73(1):190-5, 2015.

Reading, E., Walton, T. A., Liko, I., Marty, M. T., Laganowsky, A., Rees, D. C. and C. V. Robinson. The Effect of Detergent, Temperature, and Lipid on the Oligomeric State of MscL Constructs: Insights from Mass Spectrometry. Chemistry & Biology 22, 593–603, 2015.

Mamat U, Wilke K, Bramhill D, Schromm AB, Lindner B, Kohl TA, Corchero JL, Villaverde A, Schaffer L, Head SR, Souvignier C, Meredith TC, Woodard RW. Detoxifying Escherichia coli for endotoxin-free production of recombinant proteins. Microb Cell Fact. 14: 57, 2015. Erratum in: Microb Cell Fact.;14:81, 2015.

Wen-Sing Liang, Tristan C. Liu, Chun-Ju Chang, and Chorng-Liang Pan. Bioactivity of β-1,3-xylan Extracted from Caulerpa lentillifera by Using Escherichia coli ClearColi BL21(DE3)-β-1,3-xylanase XYLII. Journal of Food and Nutrition Research, vol. 3, no. 7 (2015): 437-444. doi: 10.12691/jfnr-3-7-5.

Fabián Rueda, Olivia Cano-Garrido, Uwe Mamat, Kathleen Wilke, Joaquin Seras-Franzoso, Elena García-Fruitós & Antonio Villaverde. Production of functional inclusion bodies in endotoxin-free Escherichia coli. Appl Microbiol Biotechnol. 98(22): 9229-38, 2014.

Hay, I. D. et al., In vivo polyester immobilized sortase for tagless protein purification. Microb Cell Fact  14:190, 2015.

Meeting Abstracts

Allikian K., C. Sun and S. Zhang. Expression of 2,6-sialyltransferase in Escherichia coli BL21(DE3) and endotoxin-free ClearColi® under fed-batch fermentation. Poster session presented at the 2016 SIMB Annual Meeting and Exhibition, New Orleans, LA. Abstract P24. July 2016.

Allikian K., I. Rasiah and S. Zhang. Effects of common defoaming agents on the growth of the endotoxin-free ClearColi® Escherichia coli host. Poster session presented at the 2016 SIMB Annual Meeting and Exhibition, New Orleans, LA. Abstract P22. July 2016.


Pooe, Oftense Jacob. Evaluation of the Immunomodulatory Activity of Plasmodium falciparum HSP70-1. Ph.D Thesis, University of Zululand, 2014. Note especially Figure 3.1